Difference between revisions of "Part:BBa K3971024:Design"
 
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===Design Notes===
 
===Design Notes===
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blah
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The double terminator downstream of the BFP gene had to reverse complemented (reverse complement of BBa_B0015) and the BFP gene had to be reverse complemented as well (reverse complement of BBa_K592100) since this is a bidirectional promoter, and these sequences had to be transcribed on the strand opposite to the strand with YFP.
  
  
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===Source===
 
===Source===
  
−
Thw YFP and the double terminators (coding in both directions) are present in the iGEM 2021 Distribution Kit. We plan to get the BFP gene synthesized.
+
The YFP and the double terminators (coding in both directions) are present in the iGEM 2021 Distribution Kit. We plan to get the BFP gene synthesized.
 
The promoter can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).
 
The promoter can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).
  
 
===References===
 
===References===

Latest revision as of 19:06, 20 October 2021


Promoter characterization cassette for native E.coli csc operon bidirectional promoter.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1699


Design Notes

The double terminator downstream of the BFP gene had to reverse complemented (reverse complement of BBa_B0015) and the BFP gene had to be reverse complemented as well (reverse complement of BBa_K592100) since this is a bidirectional promoter, and these sequences had to be transcribed on the strand opposite to the strand with YFP.


Source

The YFP and the double terminators (coding in both directions) are present in the iGEM 2021 Distribution Kit. We plan to get the BFP gene synthesized. The promoter can be amplified from the pCSCX plasmid, which was a gift from Claudia Vickers (Addgene plasmid # 63918 ; http://n2t.net/addgene:63918 ; RRID:Addgene_63918).

References