Difference between revisions of "Part:BBa K3739058"
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Ag43 is a surface display protein that displays the protein molecule (GFP) it carries on the cell membrane of bacteria. pelB is an exopeptide that binds to Ag43, but is unable to do its job here, secreting the protein out of the bacteria. | Ag43 is a surface display protein that displays the protein molecule (GFP) it carries on the cell membrane of bacteria. pelB is an exopeptide that binds to Ag43, but is unable to do its job here, secreting the protein out of the bacteria. | ||
| + | ===Reference=== | ||
| + | |||
| + | 1. Ahan, R. E.; Kirpat, B. M.; Saltepe, B.; Seker, U. O. S., A Self-Actuated Cellular Protein Delivery Machine. ACS Synth Biol 2019, 8 (4), 686-696. | ||
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Revision as of 17:50, 20 October 2021
J23100-B0030-pelB-Ag43-160N-his-GFP-B0010
Aly01 is a signal peptide from the alginate lyase of V.natriegens and GFP can show the secretion effciency of Aly01.
Usage and Biology
Ag43 is a surface display protein that displays the protein molecule (GFP) it carries on the cell membrane of bacteria. pelB is an exopeptide that binds to Ag43, but is unable to do its job here, secreting the protein out of the bacteria.
Reference
1. Ahan, R. E.; Kirpat, B. M.; Saltepe, B.; Seker, U. O. S., A Self-Actuated Cellular Protein Delivery Machine. ACS Synth Biol 2019, 8 (4), 686-696.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 118
Illegal NgoMIV site found at 1076
Illegal NgoMIV site found at 2300
Illegal NgoMIV site found at 2810
Illegal NgoMIV site found at 2831
Illegal AgeI site found at 1826
Illegal AgeI site found at 2570
Illegal AgeI site found at 2984
Illegal AgeI site found at 3226 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 206