Difference between revisions of "Part:BBa K3739022"
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Ag43 is a surface display protein that displays the protein molecule (GFP) it carries on the cell membrane of bacteria. pelB is an exopeptide that binds to Ag43, but is unable to do its job here, secreting the protein out of the bacteria.
 
Ag43 is a surface display protein that displays the protein molecule (GFP) it carries on the cell membrane of bacteria. pelB is an exopeptide that binds to Ag43, but is unable to do its job here, secreting the protein out of the bacteria.
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===Reference===
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1. Ahan, R. E.; Kirpat, B. M.; Saltepe, B.; Seker, U. O. S., A Self-Actuated Cellular Protein Delivery Machine. ACS Synth Biol 2019, 8 (4), 686-696.
  
 
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Revision as of 17:51, 20 October 2021


pelB-Ag43-160N-his-GFP

Ag43 is a surface display protein and GFP can show the expression effciency of Ag43


Usage and Biology

Ag43 is a surface display protein that displays the protein molecule (GFP) it carries on the cell membrane of bacteria. pelB is an exopeptide that binds to Ag43, but is unable to do its job here, secreting the protein out of the bacteria.

Reference

1. Ahan, R. E.; Kirpat, B. M.; Saltepe, B.; Seker, U. O. S., A Self-Actuated Cellular Protein Delivery Machine. ACS Synth Biol 2019, 8 (4), 686-696.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal NgoMIV site found at 1012
    Illegal NgoMIV site found at 2236
    Illegal NgoMIV site found at 2746
    Illegal NgoMIV site found at 2767
    Illegal AgeI site found at 1762
    Illegal AgeI site found at 2506
    Illegal AgeI site found at 2920
    Illegal AgeI site found at 3162
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 142