Difference between revisions of "Part:BBa K3739016"
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===Biology=== | ===Biology=== | ||
− | Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion. | + | Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.<br/> |
− | + | '''Fig.1'''. Mfp5 adhesion mechanism | |
===Usage=== | ===Usage=== | ||
− | By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter to obtain BBa_, and the constructed plasmid was transformed into BL21(DE3) to verify its expression. | + | By codon optimization and adding a 6His-tag, the sequence suitable for expression in ''BL21(DE3)'' was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter to obtain BBa_, and the constructed plasmid was transformed into ''BL21(DE3)'' to verify its expression.<br/> |
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Revision as of 19:16, 21 October 2021
AKTA-his-Mfp5
It is a kind of mucin from mussels, including AKTA expression tags and 6xHis affinity tags which can let the mucin purified by tags.
Biology
Mfp5 is a kind of mucin from mussel, which is one of the main proteins in adhesion interface. It’s side chain contains a lot of dopamine and is the main factor of adhesion.
Fig.1. Mfp5 adhesion mechanism
Usage
By codon optimization and adding a 6His-tag, the sequence suitable for expression in BL21(DE3) was constructed. The coding sequence of target gene was inserted into the expression vector with T7 promoter to obtain BBa_, and the constructed plasmid was transformed into BL21(DE3) to verify its expression.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]