Difference between revisions of "Part:BBa K3725210"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K3725210 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3725210 SequenceAndFeatures</partinfo>
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This sequence is only the promoter sequence. For the composite part with sfGFP, visit part <https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220/>.
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This sequence is only the promoter sequence. For the composite part with sfGFP, visit part <https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220>.
  
 
<!-- Uncomment this to enable Functional Parameter display  
 
<!-- Uncomment this to enable Functional Parameter display  

Revision as of 16:48, 20 October 2021


Phosphorylated NarL-Specific Promoter Sequence (pNar)

pNar is a regulatory promoter responsive to phosphorylated NarL. This gene is sourced from Bacillus Subtilis; however, it works with the DNA-binding response regulator NarL from the E. coli Nar Operon.


Usage and Biology

Lambert_GA iGEM 2021 developed a cell-free nitrate biosensor, using the pNAR promoter to quantify the amount of nitrate present in a solution. Lambert_GA’s part BBa_K3725220 is a composite part with sfGFP driven by this pNar promoter. The team’s biosensor utilized three plasmids, NarL, NarX and a sfGFP part with the pNAR promoter. This was designed by Dr. Adam Silverman at the Jewett Lab at Northwestern University and shipped to our lab for testing.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

This sequence is only the promoter sequence. For the composite part with sfGFP, visit part <https://parts.igem.org/Part:BBa_K3725220 BBa_K3725220>.