Difference between revisions of "Part:BBa K3739055"

Revision as of 21:42, 21 October 2021

J23100-B0030-LMT-PPO-his-B0010

LMT is a signal peptide from the lytic murein transglycosylase of V.natriegen and PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mfp glue.his-tag is used to be detected by anti-his antibody and purify the his-linking fusion

Biology

LMT is a signal peptide from the lytic murein transglycosylase of V.natriegen. PPO, a tyrosinase able to oxidize polyphenol, is to weaken the mussel foot protein glue. his-tag is used to be detected by anti-his antibody and purify the his-linking fusion

Usage

By codon optimization, the sequence suitable for expression in Vibrio Natriegens was constructed. Adding T7-RBS and terminator,construct new composite part BBa_K3739110.We transformed the constructed plasmid into Vibrio Natriegens to verify its successful heterologous expression.

Reference

1. Holten-Andersen, N.; Waite, J. H., Mussel-designed protective coatings for compliant substrates. J Dent Res 2008, 87 (8), 701-9.

2. Kim, E.; Dai, B.; Qiao, J. B.; Li, W.; Fortner, J. D.; Zhang, F., Microbially Synthesized Repeats of Mussel Foot Protein Display Enhanced Underwater Adhesion. ACS Appl Mater Interfaces 2018, 10 (49), 43003-43012. Sequence and Features