Difference between revisions of "Part:BBa K3861025"
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Composite part consisting of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861000">BBa_K3861000</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861010">BBa_K3861010</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Further, due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS.  
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Composite part consisting of <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861000">BBa_K3861000</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K3861012">BBa_K3861012</a>. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS. The HiBit tagged Pep8 allows to track the Pep8 in translocation assays. 
 
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Revision as of 15:39, 20 October 2021


PlldR-sicP-sptP-Pep8-HiBiT

Composite part consisting of BBa_K3861000 and BBa_K3861012. This way, the Pep8 expression is coupled to the enviromental lactate concentration mediated by PlldR. Due to the SPI T3SS secretion signal sptP and its chaperone sicP, Pep8 can be exported via the SPI T3SS. The HiBit tagged Pep8 allows to track the Pep8 in translocation assays.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 519
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
    Illegal PstI site found at 519
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 519
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 519
  • 1000
    COMPATIBLE WITH RFC[1000]