Difference between revisions of "Part:BBa K3815000"

Revision as of 15:29, 20 October 2021

Mxe GryA intein-PT-linker-ELK16 for peptide production

This part is for new purification method, ELK16 system. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tags.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 13
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 481
1000
COMPATIBLE WITH RFC[1000]

Reference

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173929/#MOESM2

@@ Line 4: / Line 4: @@
 This part is for new purification method, ELK16 system.
-This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tag.<br>
+This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tags.<br>
@@ Line 19: / Line 19: @@
 <partinfo>BBa_K3815000 parameters</partinfo>
 <!-- -->
+==Reference==
+https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6173929/#MOESM2