Difference between revisions of "Part:BBa K3815000"
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This part is for new purification method, ELK16 system.
 
This part is for new purification method, ELK16 system.
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This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.<br>
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This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tag.<br>
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Exchanging
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 15:27, 20 October 2021


Mxe GryA intein-PT-linker-ELK16 for peptide production

This part is for new purification method, ELK16 system. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein. In this system, we don't have to need the expensive column or could save time removing tag.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 13
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 481
  • 1000
    COMPATIBLE WITH RFC[1000]