Difference between revisions of "Part:BBa K3815015"

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<partinfo>BBa_K3815015 short</partinfo>
 
<partinfo>BBa_K3815015 short</partinfo>
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This is an engineered derivative of wildtype ssrA tag from <i>Escherichia coli</i>, acquired by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of <i>E.coli</i> overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.
 
  
 
==Usage and Biology==
 
==Usage and Biology==
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This is an engineered derivative of wildtype ssrA tag from Escherichia coli, acquired by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of E.coli overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.
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This is an engineered derivative of wildtype ssrA tag from <i>Escherichia coli</i>, obtained by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of E.coli overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.
 
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Revision as of 14:15, 20 October 2021


AANDENYALGA. mutant SsrA degradation tag

Usage and Biology

This is an engineered derivative of wildtype ssrA tag from Escherichia coli, obtained by mutagenizing the WT tag with mixed primers. Compared to the WT, three C-terminal amino acids LAA are replaced to LGA. To quantify its efficiency of protein degradation, ... of E.coli overnight culture bearing a plasmid expressing GFP-ssrA tag fusion protein was measured by Qubit, and then compared to the WT and other mutants.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Result



Improvement from existing parts

This is a part improved from BBa_K1051206. In this part, three C-terminal amino acids LAA were replaced with LGA, resulting in a reduced protein degradation efficiency.