Difference between revisions of "Part:BBa K3874004"

Revision as of 02:34, 21 October 2021

lactoferrin with EGFP

pEGFP-N3- lactoferrin: This gene encoding this protein is located next to a CMV promoter, and its C terminal is connected with an EGFP-tag. We changed the stop codon of lactoferrin and add an EGFP to the C terminal, so this is a new basic part.

Figure 1. The structure of EGFP-N3-plasmid.

Figure 2. The Schematic cartoon of the DNA construct of BBa_K3874004.

Figure 3. The immunofluorescence data of BBa_K3874004.

Figure 4. The western blot data of BBa_K3874004.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 pEGFP-N3- lactoferrin: This gene encoding this protein is located next to a CMV promoter, and its C terminal is connected with an EGFP-tag. We changed the stop codon of lactoferrin and add an EGFP to the C terminal, so this is a new basic part.
-<!-- Add more about the biology of this part here
+[[File:T--BJ101ID--7.png|400px|thumb|left|Figure 1. The structure of EGFP-N3-plasmid.]]
-===Usage and Biology===
+[[File:T--BJ101ID--8.png|400px|thumb|left|Figure 2. The Schematic cartoon of the DNA construct of BBa_K3874004.]]
+[[File:T--BJ101ID--9.png|400px|thumb|left|Figure 3. The immunofluorescence data of BBa_K3874004.]]
+[[File:T--BJ101ID--10.png|400px|thumb|left|Figure 4. The western blot data of BBa_K3874004.]]
 <!-- -->