Difference between revisions of "Part:BBa K3747003:Experience"

 
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===Applications of BBa_K3747003===
 
===Applications of BBa_K3747003===
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https://2021.igem.org/wiki/images/f/fb/T--Wageningen_UR--MMO_figure_7.png
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SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in <i>E. coli </i> BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure.
  
 
===User Reviews===
 
===User Reviews===

Revision as of 13:48, 20 October 2021


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K3747003

T--Wageningen_UR--MMO_figure_7.png

SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in E. coli BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure.

User Reviews

UNIQ8354d59ad902b6ec-partinfo-00000000-QINU UNIQ8354d59ad902b6ec-partinfo-00000001-QINU