Difference between revisions of "Part:BBa K3747003:Experience"
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===Applications of BBa_K3747003=== | ===Applications of BBa_K3747003=== | ||
+ | https://2021.igem.org/wiki/images/f/fb/T--Wageningen_UR--MMO_figure_7.png | ||
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+ | SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in <i>E. coli </i> BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure. | ||
===User Reviews=== | ===User Reviews=== |
Revision as of 13:48, 20 October 2021
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how you used this part and how it worked out.
Applications of BBa_K3747003
SDS-page of particulate methane monooxygenase and scaffold. pMMO mimic as described in Kim et al 2019, expressed in E. coli BL21 strain with pET-28(+)-pMMO-m1 plasmid. The lanes harbor left to right (1) PageRuler Prestained Protein Ladder product #26616. (2) Product after Sonication. (3) Product after filtered. (4) Product after His-tag purification. The expected product size is 54 kDA in all cases and is annotated with the white asterisk in the figure.
User Reviews
UNIQ8354d59ad902b6ec-partinfo-00000000-QINU UNIQ8354d59ad902b6ec-partinfo-00000001-QINU