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Latest revision as of 14:32, 20 October 2021
Interacting Guide RNA(igRNA)
The rational design of igRNA is adding additional sequences in the 5' of sgRNA.
![](https://static.igem.org/mediawiki/parts/a/a9/3-1111.png)
Usage and Biology
![](https://static.igem.org/mediawiki/parts/c/c2/3-11112.png)
Figure 1. Schematic of gRNA and igRNA.
The additional sequences include recognition sequence A, cis-repressed sequence and recognition sequence B. The cis-repressed sequence is complementary to the spacer, leading to the repression of the binding ability between igRNA with its DNA target. The recognition sequence A and the recognition sequence B are complementary to the interacting sequence A and interacting sequence B, respectively.
![](https://static.igem.org/mediawiki/parts/8/8a/3-1113.png)
The igRNA that has lower free energy, co-folding energy with target RNA as well as reduced self-comlementarity at 29℃ would be the best (Figure 2).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]