Difference between revisions of "Part:BBa K091157:Experience"

Latest revision as of 18:29, 15 May 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K091157

User Reviews

UNIQ99f19b69a88ec8e1-partinfo-00000000-QINU

No review score entered. Wideloache

After many attempts at assembly, we were not able to build this part. Perhaps some secondary structure prevents it from being cloned. See this [http://www.bio.davidson.edu/courses/genomics/2008/DeLoache/HonorsThesis.pdf document] for many information on the failed attempts at assembly. We have moved on to a different design of the promoter, in part because we discovered cross reactivity between LuxR and 3OC12. See the experience page for K091146 for more information on this cross reactivity.

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UNIQ99f19b69a88ec8e1-partinfo-00000002-QINU

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 ===Applications of BBa_K091157===
-This part was not clonable even after many attempts. We had it synthesized in conjunction with another part but when the two parts were separated, K091157 was unclonable. Perhaps some secondary structure prevents it from being cloned.
 ===User Reviews===
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 <I>Wideloache</I>
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-After many attempts at assembly, we were not able to build this part. See this [http://www.bio.davidson.edu/courses/genomics/2008/DeLoache/HonorsThesis.pdf document] for many information on the failed attempts at assembly. We have moved on to a different design of the promoter, in part because we discovered cross reactivity between LuxR and 3OC12. See the experience page for [https://parts.igem.org/Part:BBa_K091146:Experience K091146] for more information on this cross reactivity.
+After many attempts at assembly, we were not able to build this part. Perhaps some secondary structure prevents it from being cloned. See this [http://www.bio.davidson.edu/courses/genomics/2008/DeLoache/HonorsThesis.pdf document] for many information on the failed attempts at assembly. We have moved on to a different design of the promoter, in part because we discovered cross reactivity between LuxR and 3OC12. See the experience page for [https://parts.igem.org/Part:BBa_K091146:Experience K091146] for more information on this cross reactivity.
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