Difference between revisions of "Part:BBa K3989014"
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===Usage and Biology===
 
===Usage and Biology===
−
==Chracterization by team 2021 UZurich==
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====<b>Chracterization by team 2021 UZurich</b>====
 
[[File:ClyA-dCBD-SGI.png|500px]]
 
[[File:ClyA-dCBD-SGI.png|500px]]
  

Revision as of 10:31, 20 October 2021


double cellulose binding domain fused with ClyA for OMV display

In this construct, outer membrane-associated protein ClyA (Part:BBa_K811000) is used to display the double cellulose binding domain (dCBD, Part:BBa_K1321340) to the surface of bacterial outer membrane vesicles (OMVs). To make dCBD better displayed, we put multiple linkers and a 3×FLAG tag between ClyA and dCBD to minimize the steric hindrance.

Usage and Biology

Chracterization by team 2021 UZurich

ClyA-dCBD-SGI.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]