Difference between revisions of "Part:BBa K3888003"

Revision as of 11:50, 20 October 2021

HoxG1

HoxG1 coding gene with E. coli signal sequence
This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli. HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004) to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).

Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.

HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.

Sequence and Features

HoxG1 has a length of 1833bp, which is located from 5094bp to 6926bp on the plasmid diagram showed above.

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 450
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 597
Illegal BamHI site found at 1512
Illegal XhoI site found at 1834
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
Illegal AgeI site found at 1728
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3888003 short</partinfo>
-HoxG1 coding gene<br>
+HoxG1 coding gene with E. coli signal sequence<br>
 This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli. HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004) to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).