Difference between revisions of "Part:BBa K3805999"
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Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination have the effect of inhibition of targeted gene transcription. This process can silence gene expression in cells effectively. | Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination have the effect of inhibition of targeted gene transcription. This process can silence gene expression in cells effectively. | ||
| + | [[File:DCas9.jpeg|500px|center]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 10:27, 20 October 2021
sgRNA bind to T7 promoter
We designed the sgRNA (19 bp) that can bind to the T7 promoter, which can guide the dCas9 to bind to the T7 promoter and inhibit the function of the promoter.
Usage and Biology
Compared with traditional Cas9, dCas9 does not have the function of knockout, but only retains the ability to be guided into the genome by sgRNA. dCas9 can fusion or recruit transcription factors. Through the guide of sgRNA which can combine with T7 promoter specifically, dCas9 can accurately combine with the target region and regulate downstream gene transcription. The combination have the effect of inhibition of targeted gene transcription. This process can silence gene expression in cells effectively.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]