Difference between revisions of "Part:BBa K3815005"

Revision as of 10:20, 20 October 2021

NOP1v-Mxe GryA intein-PT-linker-ELK16

Descriotion of this part

Targeted protein

This part is for the purification of NOP1v. This is a small peptide that can prevent the plants from sensing ethylene by binding its receptor. In our experiment, we used it to inhibit the acceptance of ethylene that promotes the wilting of a plant and aging of cut flowers. We could not get NOP1 sufficiently when using (BBa_K3815004), so we added a valine to the N-terminus of NOP1 according to N-end-rule.

Sequence and Features

Purification system

In order to purify the peptide, we adopted ELK16 system that the past iGEM teams had not used. This part is composed of ELK16, Mxe Gyr intein, and PT linker. When this fused protein is produced, ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, the N terminal of Mxe GyrA intein is cut. Then, we can get the targeted protein.

Sequence and Features