Difference between revisions of "Part:BBa K3888003"
(→Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.) |
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
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HoxG1 has a length of 1833bp, which is located from 5094bp to 6926 on the plasmid diagram showed above. | HoxG1 has a length of 1833bp, which is located from 5094bp to 6926 on the plasmid diagram showed above. | ||
<partinfo>BBa_K3888003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3888003 SequenceAndFeatures</partinfo> | ||
Revision as of 10:15, 20 October 2021
HoxG1
HoxG1 coding gene
This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004) to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
Figure 1: The Oxygen-tolerant Hydrogenase introduction plasmid constructed, which has a length of 8261bp.
HoxG1 encodes the large subunit of oxygen-tolerant hydrogenase, and it's controlled by a Lac operator.
Sequence and Features
HoxG1 has a length of 1833bp, which is located from 5094bp to 6926 on the plasmid diagram showed above.
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 450
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 597
Illegal BamHI site found at 1512
Illegal XhoI site found at 1834 - 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450 - 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
Illegal AgeI site found at 1728 - 1000COMPATIBLE WITH RFC[1000]