Difference between revisions of "Part:BBa K4032102"
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===Design=== | ===Design=== | ||
− | The lac promoter and the double terminator are added to <partinfo>BBa_K40320xx<partinfo>, forming this part. | + | The lac promoter and the double terminator are added to <partinfo>BBa_K40320xx</partinfo>, forming this part. |
− | The lac promoter and the double terminator are used from <partinfo>BBa_J04450<partinfo>. | + | The lac promoter and the double terminator are used from <partinfo>BBa_J04450</partinfo>. |
− | This part was created by In-Fusion method using <partinfo>BBa_J04450<partinfo> and melA from E.coli K-12 as an insert. | + | This part was created by In-Fusion method using <partinfo>BBa_J04450</partinfo> and melA from E.coli K-12 as an insert. |
Revision as of 09:53, 20 October 2021
lacI+RBS+α-gal+double terminator
Contents:
・The lac promoter (from BBa_R0010)
・The RBS (from BBa_B0034)
・The E.coli alpha-galactosidase gene melA ()
・The double terminator (from BBa_B0015)
Usage and Biology
This enzyme catalyzes the hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids. https://www.uniprot.org/uniprot/P06720
Design
The lac promoter and the double terminator are added to No part name specified with partinfo tag., forming this part.
The lac promoter and the double terminator are used from BBa_J04450.
This part was created by In-Fusion method using BBa_J04450 and melA from E.coli K-12 as an insert.
Fig.1 The plasmid design of K40320xx
Experiments
Time course BL21(DE3)
Fig. 2 The growth of E.coli (BL21(DE3)) expressing of alpha-galactosidase
Pre-culture : 37 ℃, 16 h (130 rpm)
Culture : 37 ℃ (130rpm)
・Measuring OD600 every 4 hours
DH5α
Fig. 3 The growth of E.coli (DH5α)) expressing of alpha-galactosidase
Pre-culture : 34 ℃, 16 h (130 rpm)
Culture : 37 ℃ (130rpm)
・Measuring OD600 every 4 hours
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2137
Illegal AgeI site found at 2249 - 1000COMPATIBLE WITH RFC[1000]