Difference between revisions of "Part:BBa K4011002"
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===Design Considerations=== | ===Design Considerations=== | ||
| − | + | The following mutations are added in Ma_Mut: A9D, A20T, Q32H, F48S, and G62R. The last eight amino acids is a linker with the sequence EAEAEFGS. | |
| − | The following mutations are added in Ma_Mut: A9D, A20T, Q32H, F48S, and G62R. | + | |
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| − | The last eight amino acids is a linker with the sequence EAEAEFGS. | + | |
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Revision as of 09:52, 20 October 2021
Ma-Mut
Ma-Mut is an mutated protein tag derived from the protein secretion tag (Ma) of mating type alpha Saccharomyces cerevisiae. In yeast cells, proteins with the Ma tag will be secreted out of the cell. This year, we will use Ma-Mut to enable secretion of sfGFP-CBM3 and CBM3-NT2RepCT-CBM3 from yeast. This will allow for modification of our bacterial cellulose membrane produced by a co-culture of Komagataeibacter and yeast. We will construct composite parts Ma-sfGFP-CBM3 BBa_K4011010 and Ma-CBM3-NT2RepCT-CBM3 BBa_K4011011 with Ma_Mut.
Usage and Biology
Design Considerations
The following mutations are added in Ma_Mut: A9D, A20T, Q32H, F48S, and G62R. The last eight amino acids is a linker with the sequence EAEAEFGS.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]