Difference between revisions of "Part:BBa K3888003"

Revision as of 10:02, 20 October 2021

HoxG1

HoxG1 coding gene
This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004) to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011). <--!-->

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
12
INCOMPATIBLE WITH RFC[12]
Illegal SpeI site found at 450
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 597
Illegal BamHI site found at 1512
Illegal XhoI site found at 1834
23
INCOMPATIBLE WITH RFC[23]
Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
25
INCOMPATIBLE WITH RFC[25]
Illegal XbaI site found at 282
Illegal XbaI site found at 384
Illegal SpeI site found at 450
Illegal AgeI site found at 1728
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 HoxG1 coding gene<br>
-This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with [[Part:BBa_K3888004|HoxK1]] to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
+This basic part is used to achieve heterologous expression of large subunit of oxygen-tolerant Hydrogenovibrio marinus [NiFe]-hydrogenase in Escherichia coli.[1] HoxG1 encodes the large subunit of hydrogenase mentioned above, and together with HoxK1(BBa_K3888004) to form composite part Core Gene HoxG1 and HoxK1(BBa_K3888011).
-[[File:T--SJTang--BBa_K3888011-1.png]]
+[[File:pET28-pTac-lacO-hydrogenase .png]]
 <--!-->