Difference between revisions of "Part:BBa K3734008"
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                 <img width="400px" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png">
 
                 <img width="400px" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png">
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                <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                2.Caution
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                <p>GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.
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                VP16
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                <p>VP16 is a transcription activation factor, also commonly used in double yeast hybrid system. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and
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                    combine with UAS, to locate UAS.
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                </p>
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            </div>
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            <h2 class="pageContent-main__title pageContent-main__subtitle">
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                1.Pattern diagram
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                <p style="text-align: center;">
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                    <img width="400px" src="https://2021.igem.org/wiki/images/f/f3/T--CSU_CHINA--tupian8.png"></p>
 
                 <!--put image's url here-->
 
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                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p>
 
                 <p style="width: 80%;text-align:center;font-size: .9rem; margin: -1rem auto 1rem auto; color: #888;">Fig.1 The model diagram of LOV-VP16</p>

Revision as of 08:45, 20 October 2021

VP16

VP16 is a kind of protein that can activate downstream gene expression.

VP16

VP16 is a transcription activation factor, also commonly used in double yeast hybrid system. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and combine with UAS, to locate UAS.

1.Pattern diagram

Fig.1 The model diagram of LOV-VP16

2.Caution

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.

VP16

VP16 is a transcription activation factor, also commonly used in double yeast hybrid system. It can realize the activation of downstream UAS transcription. It is usually used together with GAL4 combining domain, which can recognize and combine with UAS, to locate UAS.

1.Pattern diagram

Fig.1 The model diagram of LOV-VP16

2.Caution

GAL4 combining domain is universal among eukaryotic cells, but the activation factor needs to be chosen differently according to the chassis creature.

Reference：

[1]Chun Jeih Ryu , Charles E Whitehurst, Jianzhu Chen.Expression of Gal4-VP16 and Gal4-DNA binding domain under the control of the T lymphocyte-specific lck proximal promoter in transgenic mice[J].BMB Rep. 2008 Aug 31;41(8):575-80.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]