Difference between revisions of "Part:BBa K3887004"
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pCas9 is a plamid containing Cas9 editing enzyme, used for gene modification at a specific location. Cas9 protein follows guide RNA to cut the two strands of DNA at a designated location, where strands of DNA can be edit (insertion, deletion,mutation). | pCas9 is a plamid containing Cas9 editing enzyme, used for gene modification at a specific location. Cas9 protein follows guide RNA to cut the two strands of DNA at a designated location, where strands of DNA can be edit (insertion, deletion,mutation). | ||
In our project，we used this plasmid to knockout TnaA on <i>E. coli </i> BL21(DE3) genome. Below is the map of it. | In our project，we used this plasmid to knockout TnaA on <i>E. coli </i> BL21(DE3) genome. Below is the map of it. | ||
| − | [[File:T--BJU_China--Fig.1 pCas9-1.png| | + | [[File:T--BJU_China--Fig.1 pCas9-1.png|300px|thumb|center]] |
<p class="figure-description"><b><center>Figure.1 Snapgene map of pCas9</center></b></p> | <p class="figure-description"><b><center>Figure.1 Snapgene map of pCas9</center></b></p> | ||
Revision as of 08:40, 20 October 2021
pCas
pCas9 is a plamid containing Cas9 editing enzyme, used for gene modification at a specific location. Cas9 protein follows guide RNA to cut the two strands of DNA at a designated location, where strands of DNA can be edit (insertion, deletion,mutation). In our project，we used this plasmid to knockout TnaA on E. coli BL21(DE3) genome. Below is the map of it.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 6015
Illegal NotI site found at 3034 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 8294
Illegal BamHI site found at 10296
Illegal XhoI site found at 4353 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 10131
Illegal AgeI site found at 11788 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 10113