Difference between revisions of "Part:BBa K3989024"
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<partinfo>BBa_K3989024 short</partinfo> | <partinfo>BBa_K3989024 short</partinfo> | ||
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Testing construct of the promoter P<sub>esaRC</sub>(<a href="https://parts.igem.org/Part:BBa_K3989008"> BBa_K3989008 </a>). | Testing construct of the promoter P<sub>esaRC</sub>(<a href="https://parts.igem.org/Part:BBa_K3989008"> BBa_K3989008 </a>). | ||
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===Structure=== | ===Structure=== | ||
The goal is to test the sensitivity of P<sub>esaRC</sub> to a certain AHL molecule: 3OC6HSL. See the plasmid map in figure 1. | The goal is to test the sensitivity of P<sub>esaRC</sub> to a certain AHL molecule: 3OC6HSL. See the plasmid map in figure 1. | ||
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<b>Figure 1.</b>The structure of the promoter testing construct. | <b>Figure 1.</b>The structure of the promoter testing construct. | ||
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This construct consists three parts: a constitutively expressed EsaR protein controlled by lac operon, the P<sub>esaRC</sub> promoter and a reporter GFP gene downstream. The EsaR protein can be changed by its variants(<a href="https://parts.igem.org/Part:BBa_K3989003"> BBa_K3989003 </a>, <a href="https://parts.igem.org/Part:BBa_K3989004"> BBa_K3989004 </a>, <a href="https://parts.igem.org/Part:BBa_K3989005"> BBa_K3989005 </a>) and so does the promoter(<a href="https://parts.igem.org/Part:BBa_K3989009"> BBa_K23989009 </a>). If one wants to use the promoter later after the test, the GFP can also be changed to other functional gene. | This construct consists three parts: a constitutively expressed EsaR protein controlled by lac operon, the P<sub>esaRC</sub> promoter and a reporter GFP gene downstream. The EsaR protein can be changed by its variants(<a href="https://parts.igem.org/Part:BBa_K3989003"> BBa_K3989003 </a>, <a href="https://parts.igem.org/Part:BBa_K3989004"> BBa_K3989004 </a>, <a href="https://parts.igem.org/Part:BBa_K3989005"> BBa_K3989005 </a>) and so does the promoter(<a href="https://parts.igem.org/Part:BBa_K3989009"> BBa_K23989009 </a>). If one wants to use the promoter later after the test, the GFP can also be changed to other functional gene. | ||
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===Function=== | ===Function=== | ||
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The way it works is rather simple: first we make sure the EsaR protein's existence. After the expression of the protein, it can bind to the promoter downstream and regulate the GFP expression under the control of the AHL molecule. In this case, the existence of AHL molecule will up-regulate the expression of the GFP. Thus, we can see a green fluorescence if we culture the cell containing this construct with AHL molecule. | The way it works is rather simple: first we make sure the EsaR protein's existence. After the expression of the protein, it can bind to the promoter downstream and regulate the GFP expression under the control of the AHL molecule. In this case, the existence of AHL molecule will up-regulate the expression of the GFP. Thus, we can see a green fluorescence if we culture the cell containing this construct with AHL molecule. | ||
A similar construct is designed to test the promoter P<sub>esaS</sub>(<a href="https://parts.igem.org/Part:BBa_K3989009"> BBa_K3989009 </a>). | A similar construct is designed to test the promoter P<sub>esaS</sub>(<a href="https://parts.igem.org/Part:BBa_K3989009"> BBa_K3989009 </a>). | ||
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| − | + | ===Sequence and Features=== | |
| − | === | + | <partinfo>BBa_K39890024 SequenceAndFeatures</partinfo> |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K3989024 parameters</partinfo> | <partinfo>BBa_K3989024 parameters</partinfo> | ||
Revision as of 08:15, 20 October 2021
EsaR-PesaRC-GFP
Testing construct of the promoter PesaRC( BBa_K3989008 ).
Structure
The goal is to test the sensitivity of PesaRC to a certain AHL molecule: 3OC6HSL. See the plasmid map in figure 1.
Figure 1.The structure of the promoter testing construct. This construct consists three parts: a constitutively expressed EsaR protein controlled by lac operon, the PesaRC promoter and a reporter GFP gene downstream. The EsaR protein can be changed by its variants( BBa_K3989003 , BBa_K3989004 , BBa_K3989005 ) and so does the promoter( BBa_K23989009 ). If one wants to use the promoter later after the test, the GFP can also be changed to other functional gene.
Function
The way it works is rather simple: first we make sure the EsaR protein's existence. After the expression of the protein, it can bind to the promoter downstream and regulate the GFP expression under the control of the AHL molecule. In this case, the existence of AHL molecule will up-regulate the expression of the GFP. Thus, we can see a green fluorescence if we culture the cell containing this construct with AHL molecule. A similar construct is designed to test the promoter PesaS( BBa_K3989009 ).
Sequence and Features
No part name specified with partinfo tag.