Difference between revisions of "Part:BBa K3827002"
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<h1>Characterization</h1>
 
<h1>Characterization</h1>
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[[File:T--British Columbia--plac_lldr_rfp_construct.jpg|200px|thumb|left|Lactate induction experiment of pLactate promoter in combination with lldR and a RFP gene at different lactate concentrations over a period of 18 hours.]]
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[[File:plac_lldr_rfp_construct.jpg|200px|thumb|left|Lactate induction experiment of pLactate promoter in combination with lldR and a RFP gene at different lactate concentrations over a period of 18 hours.]]
 
[[File:T--British Columbia--plac_lldr_rfp_construct.jpg]]
 
[[File:T--British Columbia--plac_lldr_rfp_construct.jpg]]

Revision as of 05:18, 20 October 2021


Lactate Inducible Reporter (pLactate + lldR + RFP)

This is a composite reporter construct made to characterize the activity of the pLactate (lactate inducible promoter) and lldR (regulatory protein) unit. It consists of the pLactate, RBS, lldR, RFP, and a terminator.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1018
    Illegal NheI site found at 1041
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 773
    Illegal AgeI site found at 885
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

Lactate induction experiment of pLactate promoter in combination with lldR and a RFP gene at different lactate concentrations over a period of 18 hours.

File:T--British Columbia--plac lldr rfp construct.jpg