Difference between revisions of "Part:BBa K3745000"
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<partinfo>BBa_K3745000 short</partinfo>
 
<partinfo>BBa_K3745000 short</partinfo>
  
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This part is a rhamnose-induced genetic circuit that contains a Rhamnose promoter, an RBS, an ORF of AmpR, and a terminator. It is improved from BBa_K1493501 by using Gibson assembly to replace the GFP with AmpR.
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===Usage and Biology===
 
===Usage and Biology===
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In our project, it is a vital part of the biosensor. After rhamnolipid is hydrolysis to rhamnose, rhamnose could induce this genetic circuit to express the ampicillin resistance gene. This mechanism increases the evolutionary fitness of the strains that have a high rhamnolipid yield in culture with ampicillin and enables the strains with favourable mutation to be automatically selected.
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Revision as of 01:32, 20 October 2021


Prha-Amp

This part is a rhamnose-induced genetic circuit that contains a Rhamnose promoter, an RBS, an ORF of AmpR, and a terminator. It is improved from BBa_K1493501 by using Gibson assembly to replace the GFP with AmpR.


Usage and Biology

In our project, it is a vital part of the biosensor. After rhamnolipid is hydrolysis to rhamnose, rhamnose could induce this genetic circuit to express the ampicillin resistance gene. This mechanism increases the evolutionary fitness of the strains that have a high rhamnolipid yield in culture with ampicillin and enables the strains with favourable mutation to be automatically selected.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 869