Difference between revisions of "Part:BBa K3792006"
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<partinfo>BBa_K3792003 short</partinfo>
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<partinfo>BBa_K3792006 short</partinfo>
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apFAB98 is a weak promoter taken from the BIOFAB collection. This device uses apFAB98 to express mRFP1, a red fluorescent protein. The apFAB98 test device consists of apFAB98, an RBS, the coding sequence for mRFP1, and a terminator.
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This test device and two others with BIOFAB promoters of different strengths were each assembled into the pSB1K3 plasmid and then transformed into E. coli NEB 5 alpha.
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The fluorescence of the cells was measured using flow cytometry. By comparing the relative fluorescence, we can determine which promoters caused higher levels of expression of mRFP1.
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The NodCIJ Generator with Weak Promoter does not produce a lot of the NodC protein, NodI protein, and NodJ protein. The NodC protein produces chitin and NodI and NodJ transports nodulation factors.
  
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3792003 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K3792006 SequenceAndFeatures</partinfo>
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This part consists of 2555 base-pair length. It includes a promoter, 3 ribosome binding sites, 3 protein-coding sequences, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used protein-coding sequences from Chitin synthase from Rhiozobium leguminosarum, Nodl a protein possibly involved in transporting chitin across the cell membrane, and NodJ, a protein also possibly involved in the transportation of chitin
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K3792003 parameters</partinfo>
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<partinfo>BBa_K3792006 parameters</partinfo>
 
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Revision as of 00:45, 20 October 2021


NodCIJ Generator I

The NodCIJ Generator with Weak Promoter does not produce a lot of the NodC protein, NodI protein, and NodJ protein. The NodC protein produces chitin and NodI and NodJ transports nodulation factors.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 492
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 854

This part consists of 2555 base-pair length. It includes a promoter, 3 ribosome binding sites, 3 protein-coding sequences, and a terminator. We used promoter K2832184, a weak promoter from the BIOFAB collection. In addition, we also used protein-coding sequences from Chitin synthase from Rhiozobium leguminosarum, Nodl a protein possibly involved in transporting chitin across the cell membrane, and NodJ, a protein also possibly involved in the transportation of chitin