Difference between revisions of "Part:BBa K3886010"

Latest revision as of 00:25, 20 October 2021

Homologous-Barcode-pBAD-Kid-Homologous

Homologous sequence attached to the 5' and 3' end of Trace and Control System (Figure 1A) were used to integrated to the E. coli fimA site using CRISPR-Cas9 system (Figure 1B) by NDNF_China iGEM 2021 team. More information about Trace and Control System, please visit part: BBa_K3886022.

Figure 1: (A)The design scheme of Trace and Control System in Hidro; (B) The gel image of the result of genome integration of Trace and Control System into fimA site.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1778
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 2030
Illegal AgeI site found at 1613
Illegal AgeI site found at 2302
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 1595

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 <partinfo>BBa_K3886010 short</partinfo>
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-<p>Homologous sequence attached to the 5' and 3' end of Trace and Control System (Figure 1A) were used to integrated to the E. coli fimA site using CRISPR-Cas9 system (Figure 1B) by NDNF_China iGEM 2021 team. More information about Trace and Control System, please visit part: BBa_K3886022.</p>
+<p>Homologous sequence attached to the 5' and 3' end of Trace and Control System (Figure 1A) were used to integrated to the E. coli fimA site using CRISPR-Cas9 system (Figure 1B) by NDNF_China iGEM 2021 team. More information about Trace and Control System, please visit part: <a href="https://parts.igem.org/Part:BBa_K3886022">BBa_K3886022</a>.</p>
 <div style="text-align: center;">
 <img src="https://2021.igem.org/wiki/images/a/a4/T--NDNF_China--part_traceandcontrol_genomeediting.png" alt="" width="700">