Difference between revisions of "Part:BBa K3886005"
Line 2: Line 2:
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K3886005 short</partinfo>
 
<partinfo>BBa_K3886005 short</partinfo>
−
 
+
<html>
 
NDNFCHINA2021 Barcode
 
NDNFCHINA2021 Barcode
  
−
<p>
+
<p>This is a part used to encode "NDNFCHINAIGEM2021“ into DNA Sequence. </p>
−
BIOT is a basic design for the expression of <i>Geobacter sulfurreducens</i>'s pilin-based electrically conductive protein nanowires (e-PNs)<sup>[1]</sup>. The system includes a tac promoter which promotes the pilin gene(<i>pilA</i>) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1A). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.</p>
+
  
 
</html>
 
</html>
 +
 
===Characterization===
 
===Characterization===
 
<html>
 
<html>

Revision as of 22:28, 19 October 2021


NDNFCHINAiGEM2021 Barcode NDNFCHINA2021 Barcode

This is a part used to encode "NDNFCHINAIGEM2021“ into DNA Sequence.

Characterization

In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system and the pilA gene to enhance the productivity and output voltage, respectively.

Sequence and Features BBa_K3886005 SequenceAndFeatures