Difference between revisions of "Part:BBa K3848009"

 
(The previous information about the species where this sequence was derived from was incorrect. This part was described as being a protein from Corynebacterium glutamicum, but BLAST analysis shows that the nucleotide sequence matches E. coli (99% similarit)
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<partinfo>BBa_K3848009 short</partinfo>
 
<partinfo>BBa_K3848009 short</partinfo>
  
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LldR (CGL2915) from Corynebacterium glutamicum is a transcription factor belonging to the GntR family, which is typically involved in the regulation of oxidized substrates associated with amino acid metabolism.  
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LldR from Escherichia coli (E. coli) is a lactate-responsive transcription factor belonging to the GntR family. LldR governs the expression of the lldPRD operon in various bacterial species including E. coli and Corynebacterium glutamicum (BBa_K4584000).
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Sensitivity of the lldR promoter can be regulated by the expression of lldP which increases the import of lactate inside the cell.
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LldR represses the expression of the lldPRD operon by binding to the O1 and O2 operator regions, in E. coli. In presence of L-lactate, the affinity of LldR to the operator regions changes, resulting in expression of lactate dehydrogenase and lactate permease.  
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Members of the GntR family are characterised by a highly conserved winged helix-turn-helix motif (wHTH) in their N-terminus domain (NTD) responsible for DNA binding. This helix-turn-helix motif is comprised of a tri-helical core, with helices 2 and 3 interacting specifically with the major groove of the DNA. Following the helical core, two beta strands are linked through a small loop, referred to as the "wing" motif. However, these transcription factors display variations in their C-terminal effector-binding and oligomerization domains. In the case of LldR, the C-terminal effector binding domain is responsible for binding to L-lactate.
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===Usage and Biology===
 
===Usage and Biology===
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Revision as of 17:58, 11 October 2023


LLDR

LldR from Escherichia coli (E. coli) is a lactate-responsive transcription factor belonging to the GntR family. LldR governs the expression of the lldPRD operon in various bacterial species including E. coli and Corynebacterium glutamicum (BBa_K4584000).

LldR represses the expression of the lldPRD operon by binding to the O1 and O2 operator regions, in E. coli. In presence of L-lactate, the affinity of LldR to the operator regions changes, resulting in expression of lactate dehydrogenase and lactate permease.

Members of the GntR family are characterised by a highly conserved winged helix-turn-helix motif (wHTH) in their N-terminus domain (NTD) responsible for DNA binding. This helix-turn-helix motif is comprised of a tri-helical core, with helices 2 and 3 interacting specifically with the major groove of the DNA. Following the helical core, two beta strands are linked through a small loop, referred to as the "wing" motif. However, these transcription factors display variations in their C-terminal effector-binding and oligomerization domains. In the case of LldR, the C-terminal effector binding domain is responsible for binding to L-lactate.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 567
  • 1000
    COMPATIBLE WITH RFC[1000]