Difference between revisions of "Part:BBa K3794000"
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There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.
 
There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.
  
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This part has been synthesised by IDT as a composite (with an upstream LacI Regulator (BBa_R0010)).  
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This part has been synthesised by IDT as a composite, which can be found at <a href="parts.igem.org/Part:BBa_K3794001>BBa_K3794001</a> (with an upstream LacI Regulator (BBa_R0010)).  
  
 
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Revision as of 18:08, 21 October 2021


PVFP-5

Perna viridis foot protein-5 (PVFP-5), is one of the first adhesive proteins secreted by the mussel foot from the Perna viridis mussel species.

PVFP-5 is an adhesive protein that can be utilised in biomedical and therapeutic applications due to its low immunogenicity and non-cytotoxicity in the body. It can be used as a bioadhesive coating for medical implants, such as a 3D printed scaffold / nerve channel for axonal regeneration.

There is an encoded N-terminal 6xHis tag and TEV cleavage site for protein purification via a Ni-NTA column. Upstream of the 6xHis tag, there is a start codon to allow for transcriptional initiation.

This part has been synthesised by IDT as a composite, which can be found at <a href="parts.igem.org/Part:BBa_K3794001>BBa_K3794001</a> (with an upstream LacI Regulator (BBa_R0010)).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]