Difference between revisions of "Part:BBa K4092013"

 
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<partinfo>BBa_K4092013 short</partinfo>
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Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C and works as a negativo control for the detection of Agave Tequilana (SST1 gene), since it has a premature stop codon that will not allow the expression of any reporter gene. It has a MFE of -31.79999924 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.RNA complementary to SST1 (sucrose 1-fructosyltransferase) from Agave tequilana.
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K4092013 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K4092013 parameters</partinfo>
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Revision as of 19:10, 19 October 2021


Control toehold for Agave tequilana (premature stop codon)

Toehold switches are RNA tools used for the detection of a sequence of interest. They're composed of four main parts: the first part is located at the beginning of the toehold and it is complementary to a given sequence of interest also called trigger; next, there is a hairpin-like sequence, which hides an RBS and start codon; subsequently, at the end of the hairpin, a linker sequence is used as a spacer before the next component; finally, a coding sequence for a reporter gene of choice is added after the toehold design (not present in this part). This toehold is functional at a temperature of 37°C and works as a negativo control for the detection of Agave Tequilana (SST1 gene), since it has a premature stop codon that will not allow the expression of any reporter gene. It has a MFE of -31.79999924 (kcal/mol) and was designed using the software Toehold Switch Creator by the Tec-Monterrey 2021 team.RNA complementary to SST1 (sucrose 1-fructosyltransferase) from Agave tequilana.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]