Difference between revisions of "Part:BBa K3794001"
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| − | This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein in E.coli. It is under the control of the <i> lac </i> promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021. | + | This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, <a href="parts.igem.org/Part:BBa_K3794000">BBa_K3794000</a>, in E.coli. It is under the control of the <i> lac </i> promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021. |
Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column. | Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column. | ||
| − | This composite part / translational unit does not contain a terminator, therefore a plasmid containing a terminator region or an addition terminator part from the iGEM registry is required. | + | This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required. |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 18:16, 21 October 2021
PVFP-5 Composite
This composite part is a translational unit for PVFP-5. It is used for expression of a 6xHis tagged PVFP-5 protein, BBa_K3794000, in E.coli. It is under the control of the lac promoter and operator. It also contains a CAP binding site. It has been codon optimised for expression in E.coli K12, and was used with pSB1A3 by KCL iGEM 2021.
Expression can be conducted using IPTG-induction, and the expressed protein can be purified using a Ni-NTA column.
This composite part / translational unit does not contain a terminator, therefore a plasmid, such as pSB1A3, containing a terminator region or an addition terminator part from the iGEM registry is required.
===Usage and Biology===
Sequence and Features