Difference between revisions of "Part:BBa K3733002"
Yuanhui Sun (Talk | contribs) |
Yuanhui Sun (Talk | contribs) |
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<partinfo>BBa_K3733002 short</partinfo> | <partinfo>BBa_K3733002 short</partinfo> | ||
| − | The NarL protein can activate transcription at many promoters include PyeaR in response to nitrate and nitrite. When the NarX protein phosphorylates by nitrate, the phosphate groups can be transferred to NarL. The phosphorylated NarL will binding the site and activate the transcription of PyeaR promoter. | + | <p>The NarL protein can activate transcription at many promoters include PyeaR in response to nitrate and nitrite. When the NarX protein phosphorylates by nitrate, the phosphate groups can be transferred to NarL. The phosphorylated NarL will binding the site and activate the transcription of PyeaR promoter.</p> |
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===Usage and Biology=== | ===Usage and Biology=== | ||
<p>The NarX/NarL two component system(TCS) can be activated if the concentration of nitrate is enough. So this NarX/NarL TCS can be designed the nitrate sensor with the PyeaR promoter(BBa_K216005). When there is enough nitrate, the NarX will change configuration and then phosphorylate. After that, the phosphorylated NarX will transport the phosphate group to NarL. Last, the phosphorylated NarL will bind to the certain sequence of PyeaR, and it acts as a transcription factor, which can active the downstream of PyeaR to transcription.</p> | <p>The NarX/NarL two component system(TCS) can be activated if the concentration of nitrate is enough. So this NarX/NarL TCS can be designed the nitrate sensor with the PyeaR promoter(BBa_K216005). When there is enough nitrate, the NarX will change configuration and then phosphorylate. After that, the phosphorylated NarX will transport the phosphate group to NarL. Last, the phosphorylated NarL will bind to the certain sequence of PyeaR, and it acts as a transcription factor, which can active the downstream of PyeaR to transcription.</p> | ||
Revision as of 15:43, 19 October 2021
EBS-PyeaR: The promoter responsive to nitrate with extra NarL binding site
The NarL protein can activate transcription at many promoters include PyeaR in response to nitrate and nitrite. When the NarX protein phosphorylates by nitrate, the phosphate groups can be transferred to NarL. The phosphorylated NarL will binding the site and activate the transcription of PyeaR promoter.
Usage and Biology
The NarX/NarL two component system(TCS) can be activated if the concentration of nitrate is enough. So this NarX/NarL TCS can be designed the nitrate sensor with the PyeaR promoter(BBa_K216005). When there is enough nitrate, the NarX will change configuration and then phosphorylate. After that, the phosphorylated NarX will transport the phosphate group to NarL. Last, the phosphorylated NarL will bind to the certain sequence of PyeaR, and it acts as a transcription factor, which can active the downstream of PyeaR to transcription.
The EBS-PyeaR is the PyeaR promoter with an extra NarL binding region (Figure 1). The extra NarL binding region is inserted to the upstream of the original region, which has the 20bp spacer. For this attempt is done, the biological function has no significantly effect. Moreover, the expression has a slight improvement.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Functional Parameters
| biology | Escherichia coli |