Difference between revisions of "Part:BBa K3815001"
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<partinfo>BBa_K3815001 short</partinfo>
 
<partinfo>BBa_K3815001 short</partinfo>
 
==Usage and Biology==
 
==Usage and Biology==
−
This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.In this part, the targeted protein is CecropinA derived from a <i>moth</i>. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.<br><br>
+
We used this part for purfication of antimicrobial peptide, CecropinA. This is derived from a <i>moth</i>. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water.
 +
This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.<br><br>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
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<h3><font size="4.5">Expression</font> </h3>
 
<h3><font size="4.5">Expression</font> </h3>
 
<ul>
 
<ul>
−
<li>Cells were grown in 1000ml LB media at 27C<sup>o</sup>.
+
<li>Cells were grown in 1000ml LB media at 27<sup>o</sup>C.
 
</ul>
 
</ul>
 
<h3><font size="4.5">Purification </font></h3>
 
<h3><font size="4.5">Purification </font></h3>

Revision as of 15:13, 19 October 2021


CecropinA-Mxe GryA intein-PT-linker-ELK16

Usage and Biology

We used this part for purfication of antimicrobial peptide, CecropinA. This is derived from a moth. This is the antimicrobial peptide that can inhibit the growth of both gram-negative bacteria and gram-positive bacteria. In our experiment, we used it to kill the bacteria in the vase water. This part is used for ELK16 system to purify the peptides. When this fused protein is produced, the part of ELK16 self-assembles and precipitates. After that, taking this aggregate and adding DTT to it, we cut the N terminal of Mxe GyrA intein. Then, we can get the targeted protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 814
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 67
    Illegal AgeI site found at 346
  • 1000
    COMPATIBLE WITH RFC[1000]

Purification

SDS-PAGE of purified peptide

Expression

  • Cells were grown in 1000ml LB media at 27oC.

Purification