Difference between revisions of "Part:BBa M10019:Design"

Revision as of 02:25, 10 May 2009

{˂FLAG˃}

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

Later

Source

pBca1256-K112602

Wobble PCR Jw0019F / Jw0020R (45 bp, EcoRI/BamHI)
Sub into pBca1256 (EcoRI/BamHI, 2472+ 910, L)
Product is pBca1256-K112602 {Flag}

Jw0019F Forward construction of Flag
cgataGAATTCatgAGATCTgactacaaggatgacgacg
Jw0020R Reverse construction of Flag
ccagtGGATCCcttgtcgtcgtcatccttgtagtc

References

Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 60:523-33

Revision as of 02:18, 10 May 2009 (view source) Johnwang (Talk \| contribs) (→‎Source) ← Older edit		Revision as of 02:25, 10 May 2009 (view source) Johnwang (Talk \| contribs) (→‎References) Newer edit →
Line 24:		Line 24:

	===References===		===References===
		+	Terpe K. (2003) Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Appl Microbiol Biotechnol. 60:523-33