Difference between revisions of "Part:BBa K4083004:Design"

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===Design Notes===
 
===Design Notes===
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https://static.igem.org/mediawiki/parts/thumb/3/3d/BBa_K4083004-NAD_synthetase_P.aeruginosa.jpg/800px-BBa_K4083004-NAD_synthetase_P.aeruginosa.jpg
 
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3D model from PDB of NH(3)-dependent NAD(+) synthetase from Pseudomonas aeruginosa [1]. The picture was made in Jmol 14.31.57.
 
  
 
===Source===
 
===Source===

Latest revision as of 08:36, 20 October 2021


nadE gene of Pseudomonas aeruginosa


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 208
    Illegal XhoI site found at 475
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 400
    Illegal NgoMIV site found at 470
    Illegal NgoMIV site found at 691
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

We obtained this coding sequence from the genome of Pseudomonas aeruginosa PAO1.

References

[1] UniProt. (n.d.). nadE - NH(3)-dependent NAD(+) synthetase - Pseudomonas aeruginosa (strain PA7) - nadE gene & protein. https://www.uniprot.org/uniprot/A6VD32

[2] RCSB PDB. (n.d.). 4XFD: Crystal Structure of a NH(3)-dependent NAD(+) synthetase from Pseudomonas aeruginosa. RCSB. https://www.rcsb.org/structure/4XFD