Difference between revisions of "Part:BBa K4083008:Design"
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===Design Notes=== | ===Design Notes=== | ||
https://static.igem.org/mediawiki/parts/4/49/BBa_K4083008-pRGPDuo2-Edited_sequence.pdf | https://static.igem.org/mediawiki/parts/4/49/BBa_K4083008-pRGPDuo2-Edited_sequence.pdf | ||
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We planned to insert rhlA and rhlB genes to MCS1 and nadE gene to MCS2 of pRGPDuo2 plasmid. Thus, the rhlA and rhlB genes are controlled by the IPTG-inducible Ptac promoter, while the nadE gene is controlled by aTc-inducible PtetR/teA promoter. Those promoters are regulated by lacI and tetR repression systems | We planned to insert rhlA and rhlB genes to MCS1 and nadE gene to MCS2 of pRGPDuo2 plasmid. Thus, the rhlA and rhlB genes are controlled by the IPTG-inducible Ptac promoter, while the nadE gene is controlled by aTc-inducible PtetR/teA promoter. Those promoters are regulated by lacI and tetR repression systems | ||
Revision as of 12:23, 19 October 2021
RPGDuo2 plasmid with nadE and rhlBA genes
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 5565
Illegal BamHI site found at 6125
Illegal XhoI site found at 1757
Illegal XhoI site found at 2024
Illegal XhoI site found at 6301 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1949
Illegal NgoMIV site found at 2019
Illegal NgoMIV site found at 2240
Illegal NgoMIV site found at 4400 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
https://static.igem.org/mediawiki/parts/4/49/BBa_K4083008-pRGPDuo2-Edited_sequence.pdf
We planned to insert rhlA and rhlB genes to MCS1 and nadE gene to MCS2 of pRGPDuo2 plasmid. Thus, the rhlA and rhlB genes are controlled by the IPTG-inducible Ptac promoter, while the nadE gene is controlled by aTc-inducible PtetR/teA promoter. Those promoters are regulated by lacI and tetR repression systems
Source
This plasmid was constructed by our team by using the pRGPDuo2 plasmid provided by Gauttam, R.