Difference between revisions of "Part:BBa K3941006"

Revision as of 11:14, 19 October 2021

pET29b+EGII (C99V)

a

Sequence and Features

Assembly Compatibility:

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 __NOTOC__
-<partinfo>BBa_K3941005 short</partinfo>
+<partinfo>BBa_K3941006 short</partinfo>
-BBa_K3941005 is a composite part that we used in our expression plasmids (pET29b). We obtain EG2 wild type with this sequence.
-This composite part contains 4 parts:
-- T7 Promoter (BBa_K3941000)<br>
-- LacO (BBa_K1624002)<br>
-- EGII (BBa_K3941001)<br>
-- T7 Terminator
-https://2021.igem.org/wiki/images/thumb/c/cd/T--Saint_Joseph--EGII-Whole.png/800px-T--Saint_Joseph--EGII-Whole.png
-<font size="-2"><b>Figure 1:</b> T7 promoter, Lac Operon, Codon optimized EGII, 6XHis and terminator</font>
-<font size="+1"><b>Codon Optimized EGII</b></font>
-This composite part originated from a codon-optimized (for E. coli DH5⍺) version of the endoglucanase (EG) gene that cleaves the internal beta-1,4-glycosidic bonds in cellulose. We optimized the sequence for expression and added a 6XHis at the end.
+a
 <!-- Add more about the biology of this part here
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 <!-- -->
 <span class='h3bb'>Sequence and Features</span>
-<partinfo>BBa_K3941005 SequenceAndFeatures</partinfo>
+<partinfo>BBa_K3941006 SequenceAndFeatures</partinfo>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
-<partinfo>BBa_K3941005 parameters</partinfo>
+<partinfo>BBa_K3941006 parameters</partinfo>
 <!-- -->