Difference between revisions of "Part:BBa K3765010"
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<partinfo>BBa_K3765010 short</partinfo> | <partinfo>BBa_K3765010 short</partinfo> | ||
| − | + | sfGFP (Superfolder GFP) is a green fluorescent protein, which unlike the normal GFP, can retain its fluorescence even when fused with poorly folding proteins, because of its robust folding. So it is often used as a fluorescent tag for large proteins. This part is an ''E. coli'' codon-optimized version of [https://parts.igem.org/Part:BBa_I746916 BBa_I746916] minus the last two amino acids. | |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part was included in our OpdA-SpyTag002 construct for easy detection of the fusion protein and also for assaying the binding between this fusion protein and the SpyCatcher002-dCBD fusion protein. | ||
| + | ---- | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3765010 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3765010 SequenceAndFeatures</partinfo> | ||
Latest revision as of 06:57, 21 October 2021
sfGFP (Superfolder GFP)
sfGFP (Superfolder GFP) is a green fluorescent protein, which unlike the normal GFP, can retain its fluorescence even when fused with poorly folding proteins, because of its robust folding. So it is often used as a fluorescent tag for large proteins. This part is an E. coli codon-optimized version of BBa_I746916 minus the last two amino acids.
Usage and Biology
This part was included in our OpdA-SpyTag002 construct for easy detection of the fusion protein and also for assaying the binding between this fusion protein and the SpyCatcher002-dCBD fusion protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 627
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]