Difference between revisions of "Part:BBa K3765007"
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<partinfo>BBa_K3765007 short</partinfo> | <partinfo>BBa_K3765007 short</partinfo> | ||
| − | + | 27 amino acid native signal peptide from the OpdA enzyme from ''Agrobacterium tumefaciens'' which helps in extracellular secretion of an attached protein into the periplasmic space. | |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part was used in our project to enable secretion of the OpdA-SpyTag002 family of fusion proteins to the periplasmic space for easier purification and minimized risk of intracellular aggregation. | ||
| + | ---- | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3765007 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3765007 SequenceAndFeatures</partinfo> | ||
Latest revision as of 06:32, 21 October 2021
OpdA Signal Peptide
27 amino acid native signal peptide from the OpdA enzyme from Agrobacterium tumefaciens which helps in extracellular secretion of an attached protein into the periplasmic space.
Usage and Biology
This part was used in our project to enable secretion of the OpdA-SpyTag002 family of fusion proteins to the periplasmic space for easier purification and minimized risk of intracellular aggregation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]