Difference between revisions of "Part:BBa K3765003"
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<partinfo>BBa_K3765003 short</partinfo> | <partinfo>BBa_K3765003 short</partinfo> | ||
− | + | Decahistidine tag which can be used for purification of tagged proteins using Ni-NTA affinity chromatography. This tag can be placed either at the N- or the C-terminus of the protein. This part is an ''E. coli'' codon-optimized version of [https://parts.igem.org/Part:BBa_K823037 BBa_K823037]. | |
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part was used in our project for purification of the SpyCatcher002-dCBD and the OpdA-SpyTag002 constructs by Ni-NTA affinity column chromatography. | ||
+ | ---- | ||
− | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3765003 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3765003 SequenceAndFeatures</partinfo> |
Latest revision as of 05:52, 21 October 2021
10xHis Tag
Decahistidine tag which can be used for purification of tagged proteins using Ni-NTA affinity chromatography. This tag can be placed either at the N- or the C-terminus of the protein. This part is an E. coli codon-optimized version of BBa_K823037.
Usage and Biology
This part was used in our project for purification of the SpyCatcher002-dCBD and the OpdA-SpyTag002 constructs by Ni-NTA affinity column chromatography.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]