Difference between revisions of "Part:BBa K3765002"
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<partinfo>BBa_K3765002 short</partinfo> | <partinfo>BBa_K3765002 short</partinfo> | ||
| − | + | 22 amino acid signal peptide which directs an attached protein to the periplasmic space in Gram-negative bacteria. This part is an ''E. coli'' codon-optimized version of [https://parts.igem.org/Part:BBa_J32015 BBa_J32015]. | |
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | This part was used in our project to enable secretion of the SpyCatcher002-dCBD fusion protein to the periplasmic space for easier purification and minimized risk of intracellular aggregation, as there was no signal sequence already present in the construct. The signal peptide gets cleaved by a signal peptidase after the protein reaches the periplasm. | ||
| + | ---- | ||
| − | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K3765002 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3765002 SequenceAndFeatures</partinfo> | ||
Latest revision as of 05:28, 21 October 2021
PelB Signal Peptide
22 amino acid signal peptide which directs an attached protein to the periplasmic space in Gram-negative bacteria. This part is an E. coli codon-optimized version of BBa_J32015.
Usage and Biology
This part was used in our project to enable secretion of the SpyCatcher002-dCBD fusion protein to the periplasmic space for easier purification and minimized risk of intracellular aggregation, as there was no signal sequence already present in the construct. The signal peptide gets cleaved by a signal peptidase after the protein reaches the periplasm.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]