Difference between revisions of "Part:BBa J70304"
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This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct. | This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct. | ||
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| + | This modified cassette is functional at 15 ug/ml chloramphenicol in Top10 E. coli cells. Slow growth and small colonies are evident on normal 35 ug/ml chloramphenicol plates. | ||
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Latest revision as of 22:06, 21 May 2009
constitutive cat cassette for Mesoplasma florum
This part is a version of J70026, the cat cassette from pC194. The original cassette is inducible, with a 5' UTR containing a hairpin structure which sequesters the Shine-Dalgarno sequence. In this mutation, we eliminate the hairpin structure from the 5' UTR to attempt a constitutive construct.
This modified cassette is functional at 15 ug/ml chloramphenicol in Top10 E. coli cells. Slow growth and small colonies are evident on normal 35 ug/ml chloramphenicol plates.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]