Difference between revisions of "Part:BBa K4033008"
Line 21: Line 21:
 
<partinfo>BBa_K4033008 parameters</partinfo>
 
<partinfo>BBa_K4033008 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
<b><font size="3">Experimental approach</font></b>
 +
 +
 +
 +
<b><font size="3">References</font></b>
 +
 +
[1] : Cummings M, Peters AD, Whitehead GFS,Menon BRK, Micklefield J, Webb SJ, et al. (2019)Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli. PLoS Biol 17(7): e3000347. https://doi.org/10.1371/journal.pbio.3000347

Revision as of 05:49, 19 October 2021


antE

AntE is extracted from Photorhabdus luminescens. It is a component of the type II PKS system that can produce octaketides in E.coli.

Biology and Usage

The entire mPKS complement from P. luminescens, comprising the KS AntD, CLF AntE, and ACP AntF, were successfully expressed as soluble recombinant proteins in E. coli. Whilst AntD is a soluble recombinant proteins in E. coli, the role of AntE as a functional heterodimer partner for AntD was unknown. Sequence features of AntE defy conventions of characterised CLFs: alignment of CLF amino acid sequences in our dataset showed AntE to fringe the clade of canonical CLFs and to also lack hallmark and gatekeeper residues that play important roles in polyketide biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Experimental approach


References

[1] : Cummings M, Peters AD, Whitehead GFS,Menon BRK, Micklefield J, Webb SJ, et al. (2019)Assembling a plug-and-play production line for combinatorial biosynthesis of aromatic polyketides in Escherichia coli. PLoS Biol 17(7): e3000347. https://doi.org/10.1371/journal.pbio.3000347