Difference between revisions of "Part:BBa K4033008"
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The entire mPKS complement from P. luminescens, comprising the KS AntD, CLF AntE, and ACP AntF, were successfully expressed as soluble recombinant proteins in E. coli. Whilst AntD is a soluble recombinant proteins in E. coli, the role of AntE as a functional heterodimer partner for AntD was unknown. Sequence features of AntE defy conventions of characterised CLFs: alignment of CLF amino acid sequences in our dataset showed AntE to fringe the clade of canonical CLFs and to also lack hallmark and gatekeeper residues that play important roles in polyketide biosynthesis. | The entire mPKS complement from P. luminescens, comprising the KS AntD, CLF AntE, and ACP AntF, were successfully expressed as soluble recombinant proteins in E. coli. Whilst AntD is a soluble recombinant proteins in E. coli, the role of AntE as a functional heterodimer partner for AntD was unknown. Sequence features of AntE defy conventions of characterised CLFs: alignment of CLF amino acid sequences in our dataset showed AntE to fringe the clade of canonical CLFs and to also lack hallmark and gatekeeper residues that play important roles in polyketide biosynthesis. | ||
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| + | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Revision as of 05:47, 19 October 2021
antE
AntE is extracted from Photorhabdus luminescens. It is a component of the type II PKS system that can produce octaketides in E.coli.
Biology and Usage
The entire mPKS complement from P. luminescens, comprising the KS AntD, CLF AntE, and ACP AntF, were successfully expressed as soluble recombinant proteins in E. coli. Whilst AntD is a soluble recombinant proteins in E. coli, the role of AntE as a functional heterodimer partner for AntD was unknown. Sequence features of AntE defy conventions of characterised CLFs: alignment of CLF amino acid sequences in our dataset showed AntE to fringe the clade of canonical CLFs and to also lack hallmark and gatekeeper residues that play important roles in polyketide biosynthesis.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]