Difference between revisions of "Part:BBa K3803016"
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<p>Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose. Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in <i style="font-style: italic ">S. cerevisiae</i>, we introduced an extra XK gene to improve the xylose utilization ability.</p> | <p>Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose. Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in <i style="font-style: italic ">S. cerevisiae</i>, we introduced an extra XK gene to improve the xylose utilization ability.</p> | ||
| + | |||
| + | <figure> | ||
| + | <img src="https://2021.igem.org/wiki/images/8/84/T--Jiangnan_China--Xylose_Pathway.png" style="width: 100%"> | ||
| + | <b>Fig.1</b> Xylose Utilization Pathway | ||
| + | </figcaption> | ||
| + | </figure> | ||
<p>For cell growth, OUC_China achieved a final OD<sub>600</sub> around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD<sub>600</sub> around 20.</p> | <p>For cell growth, OUC_China achieved a final OD<sub>600</sub> around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD<sub>600</sub> around 20.</p> | ||
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<img src="https://2021.igem.org/wiki/images/c/c7/T--Jiangnan_China--ODduibi.png" width="100%"> | <img src="https://2021.igem.org/wiki/images/c/c7/T--Jiangnan_China--ODduibi.png" width="100%"> | ||
<figcaption> | <figcaption> | ||
| − | <b>Fig. | + | <b>Fig.2</b> The OD<sub>600</sub> Value with Different Xylose Utilization Genes |
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
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<img src="https://2021.igem.org/wiki/images/8/8c/T--Jiangnan_China--HPLCXYL.png" width="100%"> | <img src="https://2021.igem.org/wiki/images/8/8c/T--Jiangnan_China--HPLCXYL.png" width="100%"> | ||
<figcaption> | <figcaption> | ||
| − | <b>Fig. | + | <b>Fig.3</b> The HPLC Results with Different Xylose Utilization Genes |
</figcaption> | </figcaption> | ||
</figure> | </figure> | ||
Revision as of 05:45, 19 October 2021
This is a efficient xylose utilization composite part.
This is an efficient xylose utilization BBa_K3803016 we built based on the parts BBa_K2314913 & BBa_K2314324 (Although they were uploaded as basic parts separately, they worked together as a composite part) from OUC_China in 2017 iGEM.
Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose. Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in S. cerevisiae, we introduced an extra XK gene to improve the xylose utilization ability.
Fig.1 Xylose Utilization Pathway
For cell growth, OUC_China achieved a final OD600 around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD600 around 20.
For our own product yield, our production also improved after substituting our three-gene cassette for OUC_China's two-gene one. The improved production was very obvious after the parts improvement based on our HPLC results.
For more information about our parts and improvement, you can see details here
Reference:
[1] Construction of efficient xylose-fermenting Saccharomyces cerevisiae through a synthetic isozyme system of xylose reductase from Scheffersomyces stipitis. Bioresource Technology, Jung-Hyun Jo, Yong-Cheol Park, Yong-Su Jin, Jin-Ho Seo, Bioresource Technology, 241 (2017) 88–94.
[2] Metabolic engineering of Saccharomyces cerevisiae for production of Shinorine, a sunscreen material, from xylose. Seong-Hee Park, Kyusung Lee, Jae Woo Jang and Ji-Sook Hahn, ACS Synthetic Biology, 2019 (8), 346−357.
Sequence and Features