Difference between revisions of "Part:BBa K3803016"
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<partinfo>BBa_K3803016 short</partinfo>
 
<partinfo>BBa_K3803016 short</partinfo>
  
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Based on the parts (BBa_K2314913 & BBa_K2314324) from OUC_China in 2017 iGEM, we built a more efficient xylose utilization part BBa_K3803016.
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Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose.Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in S. cerevisiae, we introduced an extra XK gene to improve the xylose utilization ability.
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<p>This is an efficient xylose utilization BBa_K3803016 we built based on the parts <a href="">BBa_K2314913</a> & <a href="">BBa_K2314324</a> (Although they were uploaded as basic parts separately, they worked together as a composite part) from OUC_China in 2017 iGEM.</p>
  
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For cell growth, OUC_China achieved a final OD600 around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD600 around 20.
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<p>Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose. Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in <i style="font-style: italic ">S. cerevisiae</i>, we introduced an extra XK gene to improve the xylose utilization ability.</p>
  
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For our own product yield, our production also improved after substituting our three-gene cassette for OUC_China's two-gene one. The improved production was very obvious after the parts improvement based on our HPLC results.
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<p>For cell growth, OUC_China achieved a final OD<sub>600</sub> around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD<sub>600</sub> around 20.</p>
  
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For more information about our parts and improvement, you can click here: https://2021.igem.org/Team:Jiangnan_China/Improve
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<figure>
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<img src="https://2021.igem.org/wiki/images/c/c7/T--Jiangnan_China--ODduibi.png" width="90%">
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<figcaption>
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<b>Fig.1</b> The OD<sub>600</sub> Value with Different Xylose Utilization Genes
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</figure>
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<p>For our own product yield, our production also improved after substituting our three-gene cassette for OUC_China's two-gene one. The improved production was very obvious after the parts improvement based on our HPLC results.</p>
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<figure>
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<img src="https://2021.igem.org/wiki/images/8/8c/T--Jiangnan_China--HPLCXYL.png" width="70%">
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<figcaption>
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<b>Fig.1</b> The HPLC Results with Different Xylose Utilization Genes
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</figcaption>
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</figure>
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<p>For more information about our parts and improvement, you can click our <a href="https://2021.igem.org/Team:Jiangnan_China/Improve">page</a>. </p>
  
 
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<!-- Add more about the biology of this part here

Revision as of 05:36, 19 October 2021


This is a efficient xylose utilization composite part.

This is an efficient xylose utilization BBa_K3803016 we built based on the parts BBa_K2314913 & BBa_K2314324 (Although they were uploaded as basic parts separately, they worked together as a composite part) from OUC_China in 2017 iGEM.

Xylose reductase (XR) will first transform xylose to xylitol, and xylitol dehydrogenase (XDH) can further convert xylitol to xylulose. Then, xylulose will be converted to xylulose 5-phosphate (X5P) by the native xylulose kinase (XK). Apart from the original XR and XDH genes, considering the low copy number of native XK gene in S. cerevisiae, we introduced an extra XK gene to improve the xylose utilization ability.

For cell growth, OUC_China achieved a final OD600 around 2.35 in YNB-based xylose media. After our improvement on their parts, we could get a final OD600 around 20.

Fig.1 The OD600 Value with Different Xylose Utilization Genes

For our own product yield, our production also improved after substituting our three-gene cassette for OUC_China's two-gene one. The improved production was very obvious after the parts improvement based on our HPLC results.

Fig.1 The HPLC Results with Different Xylose Utilization Genes

For more information about our parts and improvement, you can click our page.

Sequence and Features BBa_K3803016 SequenceAndFeatures