Difference between revisions of "Part:BBa K3771076"

Revision as of 01:35, 22 October 2021

PlacI-CSAD

Description

P_lacI-csad is a composite part consisting of the lacI promoter and the csad sequences. This part was used in in vivo testing of taurine production. The sequence for csad enzyme and lacI promoter were ligated and transformed into E. coli to calculate taurine production using high-performance liquid chromatography (HPLC).

Biology

lacI promoter constitutively facilitates the expression of CSAD enzyme.

Fig. 1 L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase

Usage

Characterization

pSAA-P_LacI-csd

Fig. 2 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)

Fig. 3 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)

References
Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 476
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 261

@@ Line 18: / Line 18: @@
 <div style="width=100%; display:flex; align-items: center; justify-content: center;">
 <img src="https://2021.igem.org/wiki/images/c/c9/T--NCKU_Tainan--taurine_pathway_1.png" style="width:50%;">
-</div>
+<p align="center">Fig. 1 L-cysteine sulfonic acid synthase catalyzes the reaction that turns O-phospho-L-serine into L-cysteine sulfonic acid. CDO1, L-cysteine dioxygenase; CSAD, L-cysteine sulfinic acid decarboxylase</p>
+ </div>
@@ Line 27: / Line 29: @@
 <br>
-<br>pSAA-pLacI-CSAD<br>
+  <br>pSAA-<i>P<sub>LacI</sub>-csd</i><br>
 <div style="width=100%; display:flex; align-items: center; justify-content: center;">
 <img src="https://2021.igem.org/wiki/images/8/86/T--NCKU_Tainan--CSAD-PCR.png" style="width:35%;">
 </div>
-<p align="center">Fig. 1 Confirmation of csad fragment by PCR. M: Marker; Lane 1: csad (1368 bp)</p>
+  <p align="center">Fig. 2 Confirmation of <i>csad</i> fragment by PCR. M: Marker; Lane 1: <i>csad</i> (1368 bp)</p>
@@ Line 38: / Line 40: @@
 <img src="https://2021.igem.org/wiki/images/4/48/T--NCKU_Tainan--CSAD1-Vactor-digestion.png" style="width:35%;">
 </div>
-<p align="center">Fig. 2 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p>
+<p align="center">Fig. 3 Confirmation of pSAA fragment by digestion. M: Marker; Lane 1: pSAA (2531 bp)</p>